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- 保存条件:
常温,避光
- 克隆性:
单克隆
- 抗体名:
SERPINA3C / Serpina3c抗体
抗体类型: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
抗体宿主 : Rabbit IgG
缓冲液 : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
制备方法 : Produced in rabbits immunized with purified, human cell-derived, recombinant mouse SERPINA3C extracellular domain ( rM Serpina3c ; Catalog#50375-M08H ; NP_032484.1 ; Met 1 - Ala 417 ). Total IgG was purified by Protein A affinity chromatography.
SERPINA3C / Serpina3c抗体背景综述
Serpins are the largest and most diverse family of serine protease inhibitors which are involved in a number of fundamental biological processes such as blood coagulation, complement activation, fibrinolysis, angiogenesis, inflammation and tumor suppression and are expressed in a cell-specific manner. Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. The acronym serpin was originally coined because many serpins inhibit chymotrypsin-like serine proteases (serine protease inhibitors). Over 1000 serpins have been identified. Serine protease inhibitor A3C, also known as Kallikrein-binding protein, KBP, and Serpina3c, is a secreted protein which belongs to the serpin family. The reactive center loop (RCL) extends out from the body of the Serpina3c protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the serpin reactive site and the protease. The resulting inactive serpin-protease complex is highly stable. Variability within the reactive center loop (RCL) sequences of Serpina3 paralogs may determine target protease specificity.
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文献和实验Clonality - X Chromosome Inactivation Assay
can utilize X chromosome inactivation (methylation) to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome
珠子对照样品外每个样品中加入一抗,抗体的量按照以往经验来加,1-10ug的抗体/25ugDNA效果较好。3加入20ul的proteinA/G珠子(预先用超声处理成为单链的鲱精DNA和BSA吸附,详见步骤4.3a)到所有样品总,4°C摇转IP过夜。3a将蛋白A/G珠子与单链鲱精DNA进行预处理。如果同时使用蛋白A珠子和蛋白G珠子,等体积混合这两种珠子使用RIPA溶液洗3次。除去RIPA溶液,加入单链鲱精DNA到珠子终浓度为75ng/μl,再用BSA将珠子终浓度定到0.1μg/μl。加入两倍珠子
人转录因子E2F1 ( E2F1 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E2F1 单抗包被于酶标板上,标准品和样品中的 E2F1与单抗结合,加入生物素化的抗人 E2F1 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸,在 450nm 处测 OD 值,E2F1 浓度与 OD 值成正比
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