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- 保存条件:
常温,避光
- 克隆性:
单克隆
- 抗体名:
SerpinB8 / CAP2抗体
Antibody Type : Rabbit Polyclonal Antibody ( Antibody Purification Platform )
抗体宿主 : Rabbit IgG
缓冲液 : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
制备方法 : Produced in rabbits immunized with purified, human cell-derived, recombinant mouse SerpinB8 ( rM SerpinB8 ; Catalog#50215-M08B ; NP_035589.1 ; Met 1 - Pro 374 ). Total IgG was purified by Protein A affinity chromatography
SerpinB8 / CAP2抗体背景综述
Serpins are the largest and most diverse family of serine protease inhibitors which are involved in a number of fundamental biological processes such as blood coagulation, complement activation, fibrinolysis, angiogenesis, inflammation and tumor suppression and are expressed in a cell-specific manner.
Mouse SerpinB8, also known as Cytoplasmic antiproteinase 2, Peptidase inhibitor 8, SerpinB8, PI-8, SERPINB8 and CAP2, is a member of the Serpin superfamily. SERPINB8 was broadly expressed. In normal neuroendocrine tissues, strongest SerpinB8 expression was detected in islets of Langerhans of the pancreas. Moderate SerpinB8 expression was observed in neuroendocrine cells of the thyroid, adrenal cortex, colon, and pituitary gland. In the pancreas, SerpinB8 is specifically expressed by insulin-producing beta cells, and can be used as an additional diagnostic immunohistochemical marker. Mouse SerpinB8 distribution alters during kidney regeneration, possibly to control a prohormone convertase involved in inflammation or tissue repair.
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xy-2100xy EPEC/E.coli(enteropathogenic E.coli,EPEC)普通大肠埃希菌菌体蛋白(非致病性)
xy-1763xy EphA1 R(Ephrin A1 Receptor)EphA1-R受体抗原
xy-1424xy EPOR peptide红细胞生成素受体抗原
xy-1522xy phospho-ERK1/MAPK-1/2(pThr183 + pTyr185)磷酸化丝裂原活化蛋白激酶1/2抗原
xy-0996xy ERK(Extracellular signal-regulated kinase)丝裂原活化蛋白激酶抗原
xy-1020xy MAPK1/ERK3 (Mitogen-activated protein kinase 3; P44MAPK)丝裂原活化蛋白激酶3抗原
xy-1319xy MAPK4(Mitogen-activated protein kinase 4)丝裂原活化蛋白激酶4抗原
xy-0894xy ER- Alpha(phospho-Ser167)peptide人磷酸化雌激素受体α多肽抗原
xy-1643xy ER- Alpha(phospho-Tyr537)peptide磷酸化雌激素受体α抗原
xy-0253xy ER- Alpha /ER alpha peptide雌激素受体α抗原
xy-2098xy ER- Alpha/ER alpha peptide雌激素受体α抗原
xy-0255xy ER- Beta (Estrogen receptor-beta) peptide雌激素受体β抗原
xy-1285xy ets1/E26 (E-twenty six 1)Ets转录因子家族ets1抗原
xy-0983xy EV71(Human enterovirus 71)VP1(CT)肠道病毒71型/手足口病病毒抗原(C端)
xy-2221xy ATEXPA10 peptide植物扩张蛋白ATEXPA10(拟南芥)抗原
xy-1343xy Ezrin/Radixin埃兹蛋白(多肽)
xy-0949xy F1 operon positive regulatory protein(NT)鼠疫耶尔森氏菌F1荚膜蛋白(N端多肽)
xy-0950xy F1 operon positive regulatory protein(CT)鼠疫耶尔森氏菌F1荚膜蛋白(C端多肽)
xy-2135xy FABP(brain) (Fatty acid-binding protein brain)脑型脂肪酸结合蛋白抗原
xy-0511xy FADD (Fas-associated protein with death domain)Fas死亡结构域相关蛋白抗原
xy-1340xy FAK(Focaladhesin kinase)粘着斑激酶抗原
xy-1639xy phospho-FAK(pTyr577)磷酸化粘着斑激酶抗原
xy-1642xy phospho-FAK(pSer732)(phospho-Focal adhesion kinase)磷酸化粘着斑激酶抗原
xy-1765xy FAST kinase(Fas-activated serine/threonine kinase)Fas活化的丝/苏氨酸激酶抗原
xy-1157xy FBN1 (fibrillin 1)原纤维蛋白1抗原
xy-1256xy FGF4/HSTF1/HBGF-4(Fibroblast Growth Factor4)纤维母细胞生长因子4抗原
xy-1257xy FGF5 peptide纤维母细胞生长因子5抗原
xy-0809xy Fibulin 1衰老关键蛋白抗原
xy-1170xy FOS BFosB抗原
xy-1188xy FRA2/FOSL2(Fos related/like antigen 2)FOS样抗原2
xy-2585xy FOXF1(Forkhead box protein F1)叉头蛋白F1抗原
xy-1775xy FOXJ1/HFH-4 peptide插头蛋白J1抗原
xy-1275xy FoxP1(Forkhead box P1)FoxP1(T细胞转录因子)抗原
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文献和实验脾细胞与小鼠骨髓瘤细胞(P3-X63/Ag8)融合,融合的细胞既获得了亲代脾细胞分泌特异性抗体的特性,又具有骨髓瘤细胞大量繁殖的能力,成为一种既能分泌特异性抗体又能长命的杂交瘤细胞。该技术为抗体的分子生物学研究提供了全新的手段。极大地促进了免疫学,遗传学,分子生物学的快速发展。 但传统抗体目前也面临诸多问题,如: 每使用一管新抗体都需要进行滴定测试; 多种同型对照,实验设计繁琐; 需要添加Fc阻断试剂; 抗体不佳,阳性细胞群不明显; 抗体作为细胞生物学和生物化学中最重要的试剂之一,科学界每年在这类
人转录因子E2F1 ( E2F1 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E2F1 单抗包被于酶标板上,标准品和样品中的 E2F1与单抗结合,加入生物素化的抗人 E2F1 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸,在 450nm 处测 OD 值,E2F1 浓度与 OD 值成正比
luozhifeng339 请教各位达达,我准备做EMSA,如果做SUPER-SHIFT的话,该选择原装抗体还是分装的抗体呢,盼不吝赐教! wanglinling 看到一篇EMSA的文章,不知道里面有没有回答你的问题哈,不过英文的值得阅读哈。 GEL SUPER-SHIFT ASSAY/ EMSA Materials/Reagents: See Gel-shift assay Procedure
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