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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温,避光
- 克隆性:
单克隆
- 抗体名:
Decorin / DCN抗体
Antibody Type : Rabbit Polyclonal Antibody ( Antibody Purification Platform )
抗体宿主 : Rabbit IgG
缓冲液 : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
制备方法 : Produced in rabbits immunized with purified, human cell-derived, recombinant human Decorin ( rh Decorin ; Catalog#10189-H08H ; NP_001911.1 ; Met 1 - Lys 359 ). Total IgG was purified by Protein A affinity chromatography
Decorin / DCN抗体背景综述
Decorin is a ubiquitous small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a core protein and a covalently linked glycosaminoglycan chain which is either chondroitin sulfate (CS) or dermatan sulfate (DS). As a component of connective tissue, decorin interacts with several extracellular matrix components, such as type I collagen and fibronectin, and plays a role in matrix assembly. The decorin core protein also binds to growth factors including TGF-beta, and to the decorin endocytosis receptor. Decorin is capable of suppressing the growth and the metastasis of a wide range of cancer cells in vitro through attenuation of the EGFR -mediated intacellular signaling and induction of apoptosis. Multiple alternatively spliced transcript variants for the decorin gene have been described. Mutations or deficiency of the decorin gene are associated with Marfan syndrome.
Decorin / DCN抗体相关产品如下:xy-0114xy Aromatase芳香化酶(抗原)
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xy-2353xy Mast Cell Chymase肥大细胞类糜蛋白酶1
xy-2373xy Ceruloplasmin peptide铜蓝蛋白抗原
xy-2394xy CKLF1趋化素样蛋白
xy-0055xy ART(Artemin)(抗原)
xy-1510xy AATK peptide细胞凋亡关联酪氨酸激酶抗原
xy-0261xy AQP-2 (aquaporin Protein-2)水通道蛋白-2(抗原)
xy-1627xy ABCA1(ATP binding cassette transporter A1)腺苷三磷酸结合盒转运体A1抗原
xy-2535xy TFPI/LACI组织因子途径抑制剂
xy-2540xy GLUT12葡萄糖转运蛋白12
xy-2558xy EGFRvIII表皮生长因子受体III型突变体抗原
xy-2570xy CD26CD26抗原
xy-1231xy ABCG1 peptide三磷酸腺苷结合盒亚家族G1抗原
xy-2804xy Cardiac Troponin T心肌特异性肌钙蛋白T
xy-0164xy BACE( ASP2 ) β分泌酶(抗原)
xy-0583xy ABL1(v-abl Abelson murine leukemia viral oncogene homolog 1)ABL1抗原
xy-2940xy FUCA1/Alpha L fucosidase I peptideα-L岩藻糖苷酶抗原
xy-0275xy Bassoon(BSN)
xy-0127xy Bax peptideBax(多肽抗原)
xy-0409xy-FITxy Leptin peptide/FITC荧光素FITC标记瘦素抗原
xy-0032xy Bcl-2(抗原)Bcl-2(多肽抗原)
xy-0248xy BDNF (Brain-derived Neurotrophin factor)脑源神经营养因子(脑衍化神经营养因子)(多肽片断抗原)
xy-2249xy ACA11 peptide拟南芥ACA11蛋白多肽抗原
xy-2247xy AHA3 peptide植物蛋白AHA3抗原(拟南芥)
xy-0217xy bFGF碱性成纤维细胞生长因子(多肽抗原)
xy-1004xy ACE2(Angiotensin converting enzyme 2)血管紧张素转换酶2抗原
xy-0044xy BNP脑钠素(多肽抗原)
xyb-0044xy BNP/Biotin生物素化脑钠素多肽
xy-0324xy C5过敏毒素C5(补体C5)抗原
xy-0091xy CA125卵巢癌抗原
xy-0043xy CAD(Caspase-Activated Deoxyribonuclease)Caspase 激活的脱氧核糖核酸酶(抗原)
xy-3550xy MYOG肌红蛋白抗原
xy-0095xy Calponin 1钙调节蛋白-1(抗原)
xy-3679xy COMP软骨寡聚基质蛋白抗原
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文献和实验脾细胞与小鼠骨髓瘤细胞(P3-X63/Ag8)融合,融合的细胞既获得了亲代脾细胞分泌特异性抗体的特性,又具有骨髓瘤细胞大量繁殖的能力,成为一种既能分泌特异性抗体又能长命的杂交瘤细胞。该技术为抗体的分子生物学研究提供了全新的手段。极大地促进了免疫学,遗传学,分子生物学的快速发展。 但传统抗体目前也面临诸多问题,如: 每使用一管新抗体都需要进行滴定测试; 多种同型对照,实验设计繁琐; 需要添加Fc阻断试剂; 抗体不佳,阳性细胞群不明显; 抗体作为细胞生物学和生物化学中最重要的试剂之一,科学界每年在这类
人转录因子E2F1 ( E2F1 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E2F1 单抗包被于酶标板上,标准品和样品中的 E2F1与单抗结合,加入生物素化的抗人 E2F1 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸,在 450nm 处测 OD 值,E2F1 浓度与 OD 值成正比
luozhifeng339 请教各位达达,我准备做EMSA,如果做SUPER-SHIFT的话,该选择原装抗体还是分装的抗体呢,盼不吝赐教! wanglinling 看到一篇EMSA的文章,不知道里面有没有回答你的问题哈,不过英文的值得阅读哈。 GEL SUPER-SHIFT ASSAY/ EMSA Materials/Reagents: See Gel-shift assay Procedure
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