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文献和实验Analysis of Proteins using Small Format 2D Gel Electrophoresis
onto blotting paper using a heated gel dryer. Stained gels, not to be processed for autoradiography, are washed in excess tap water and then soaked in 0.5% (v/v) glycerol for 60 minutes prior to being dried between cellophane sheets in a heated air dryer.
Agarose Gel Electrophoresis of DNA
gel. 2) Cast the gel with the comb in place. 3) Add 6X gel loading buffer to sample and load the samples into wells. 4) Run the gel submerged in 1X TAE or TBE (30-60 min. at 100-150V) 5) Stain the gel with ethidium bromide solution (10 µl
Gel-elongation assay for type II fatty acid s
Gel-elongation assay for type II fatty acid synthesis Srinivas Kodali Andrew Galgoci Sheo Singh Dr. Jun Wang Dr., jun_wang2@merck.com, Merck Research Laboratories
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