3-AMINOPROPYL-FUNCTIONALIZED S

Conjugation of 5′‐Functionalized Oligodeoxyribonucleotides with Properly Functionalized Ligands

2‐mL vial equipped with Teflon‐faced septum Analytical silica gel TLC plates (e.g., 5554 Kieselgel 60F plates; Merck

Silica gel吸附法分离与纯化DNA片段

药品试剂：经BamHI 及HindIII 作用的pBlueGus 及pQE31含EtBr 的1.2% 12-well agarose gel1×TAE 电泳缓冲液QIAquick Gel Extraction Kit （Qiagen），内含：QIAquick spin column （管柱底部为silica gel membrane）Buffer QGBuffer PEBuffer EB （10 mM Tris-HCl, pH 8.5）2 mL 收集管TE-8.0方法步骤：1） 经BamHI

Silica Clean-up of DNA

and eluted from silica with 30μl 1X restriction buffer •Procedure useful for cleaning DNA from PCR reactions,restriction digests,etc.as well as purifying from agarose gel slices •If 6 M NaI solution becomes yellow,it will still bind DNA if 1/200 volume 10