HYDRANAL-BUFFER BUFFER CAPACIT

Preparation of Microinjection Buffer

EDTA, pH 8.0 (autoclaved) 100 mM NaCl 1 ml of 5 M NaCl (autoclaved) 1x Polyamines 50 microliters 1000x Polyamines mix Autoclaved H2O up to 50 ml NOTE: Prepare fresh and discard unused microinjection buffer.

10X TBE BUFFER

for 20 min 1 Liters 108g Tris base 55g Boric acid 40mls 0.5M EDTA (pH 8.0) autoclave for 20 min   上一篇：Zymolyase (3mg/ml)   下一篇：20X SSPE BUFFER

CCMB buffer to make chemical competent cell

the fragile cells much easier to resuspend It is often easier to resuspend pellets by mixing before adding large amounts of buffer Gently resuspend in 80 ml of ice cold CCMB80 buffer sometimes this is less than completely gentle. It still works. Incubate