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文献和实验Any chromatographic procedure for the separation of phosphoinositols will require a supply of standards, both for validation of the technique and identification of the products. Tritium-radiolabeled standards are commercially available in 1–
The [14C]Deoxyglucose Method for Measurement of Local Cerebral Glucose Utilization
The brain is a complex, heterogeneous organ composed of many anatomical and functional components with markedly different levels of functional activity that vary independently with time and function. Other tissues are generally far
Proliferation Assay: [3H] Thymidine incorporation
cells for 2-4 hours then treat. 5) Six-twenty four hours before cells are to be harvested, add 1 µCi/ml [3H]-TdR to each well. Harvesting: 6) Wash cells 2 times with ice cold PBS. 7) Wash cells 2 times with 5% TCA.
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