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文献和实验AA Metabolite Quantitation of Media Post-AA Labeling
TLC plate for TLC system #1, use 20µl to spot a linear K TLC plate for TLC system #2. TLC : A) Allow TLC tanks to equilibrate 1-2 hrs with solvent prior to running. SYSTEM #1 --> SeparatesPA, TXB2, HHT, HETE & AA. ethylacetate
AA Metabolite Quantitation of Cell Pellets Post-AA Labeling
lipids in 60µl of chloroform. 12) Use 20µl to spot a linear K TLC plate for TLC system #1, use 20µl to spot a linear K TLC plate for TLC system #2. TLC : A) Allow TLC tanks to equilibrate 1-2 hrs with solvent prior to running
AA & Metabolite Quantitation of Media Post-AA
AA & Metabolite Quantitation of Media Post-AA Labeling Contributor: Suprya Jayadev Date: November 4, 1994 1) Remove 2 500 µl aliquots of supernatant into scintillation vials, add scintillation fluid and count
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