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文献和实验Transformation of E. coli by Electroporation
Chamber. 7. Set the resistance on the Voltage Booster to 4 kΩ; set the Pulse Control unit to LOW and 330 FF; double check connections. 8. Charge the Pulse Control unit by setting the CHARGE ARM switch on the Pulse Control unit to CHARGE
一、TUNEL法的实验原理 细胞发生凋亡时, 染色体DNA双链或单链断裂产生大量的粘性3'-OH末端,可在脱氧核糖核苷酸末端转移酶(TdT)的作用下,将脱氧核糖核苷酸和荧光素、过氧化物酶、碱性磷酸酶或生物素形成的衍生物标记到DNA的3'-末端,从而可进行凋亡细胞的检测,这类方法称为脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(terminal -deoxynucleotidyl transferase mediated nick end labeling, TUNEL)。由于正常的或正在增殖
Two-Phase Extraction of Proteins from Cell Debris
diagram that represents compositions at which cell disintegrate and nucleic acids partition strongly to the lower phase, (here, the potassium phosphate-rich phase), and the target protein is recovered with a desired yield in the upper phase, (here, the PEG-
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