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文献和实验TRICINE GELS Protocol (For Low MW proteins (
% Acrylamide solution 19:1 (Acrylamide 19 / Methylene bis Acrylamide 1) for short peptides. Keep 4°C. 3) Glycerol 70% 4) Cathode buffer 10X (upper buffer): 1M Tris + 1M Tricine + 1% SDS pH 8.25. Keep RT. (Prepare: 24.2gr Tris + 35.84gr Tricine + 2gr
DNA Interstrand Cross‐Link Formation Using Furan as a Masked Reactive Aldehyde
, is described. The methods allow gram?scale synthesis starting from stable and readily available furan derivatives. Cross?linking requires selective oxidation of the furan moiety to an aldehyde. The masked nature of the latter avoids undesired and off?target
Protocols for Peptidomic Analysis of Spider Venoms
. Proteomics profiling of spider venoms (the components with MW >10 kDa) could be achieved through conventional 2-DE-based proteomics methods combined with MS or MS/MS detection. Peptidomic profiling (of the components with MW below ~10 kDa) is usually achieved
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