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- 2025年11月02日
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文献和实验Fluorescein labeling of proteins
Phosphate buffered saline,pH 7.4 * Protein to be labeled,purified,~1 mg/ml in PBS * Column for gel filtration,e.g.10 ml Sephadex G-10 column * Dialysis tubing,10,000 MW cutoff * Centricon microconcentrators (Amicon) Method Prepare
Fluorescein labeling of proteins
filtration, e.g. 10 ml Sephadex G-10 column * Dialysis tubing, 10,000 MW cutoff * Centricon microconcentrators (Amicon) Method Prepare or otherwise obtain pure protein; make sure it is free of other contaminating proteins (e.g. albumin
LABELING GC-GLOBULIN WITH FLUORESCEIN
in the void volume. 7.Concentrate with Centricon-30. 8.Clarify in a 42.2Ti rotor at 25,000 rpm,4oC for 30 min.Measure concentration with Lowry assay. 9.Dialyze into PBS solution A,can be stored at 4℃ for several weeks with 0.01% NaN3,or frozen in liquid N2.
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