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文献和实验PEGPEI Copolymers for Oligonucleotide Delivery to Cells and Tissues
compounds that markedly improve the in vitro and in vivo delivery of AOs to cells and tissues. By varying the Mw of PEI, as well as the nature of PEG shielding, PEG-PEI-AO nanoparticulates can be prepared with a dynamic range of size, surface charge
[M]=A259 /15.4x103 =A259 1:2000 x0.130 adjust the final volume. Betaine monohydrate C5 H11 NO2 xH2 O; Mw=135.2g/M; (store at 4o C). Conc. Stock %(w/w) 100ml 200ml 700ml Betaine 135.2g/M 5M
2 EDTA800 mLType I water?/span> 20.5 mL10 N NaOH to pH 7.4x mLType I water1000 mLAutoclave or filter sterilize. SSPE can replace SSC plus NaPO4 , pH 6.7.50% Dextran sulfate MW 5000Dextran sulfate MW 5000 works just as well as the traditional MW 500
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