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Phospho-MKK3(Ser218)/MKK6(Ser2

07) Antibody
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  • ¥2400
  • PLLABS已认证
  • 加拿大
  • PL0304506
  • 2025年07月06日
  • WB: 1:100-500 E: 1:500-1000 IP: 1:20-100 IHC: 1:100-500 IF: 1:100-500
  • Rabbit
  • Hu, Mo, Rat, Chi, Dog, Pig, Cow, Hor, ,
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      PhosphoPeptide derived from human MKK3 around the phosphorylation site of Ser218.

    • 亚型

      Rabbit Polyclonal IgG

    • 形态

      液态

    • 保存条件

      保存于-20℃

    • 克隆性

      多克隆

    • 标记物

      无标记(PLlabs可供应生物素、酶、荧光素标记的抗体)

    • 适应物种

      Hu, Mo, Rat, Chi, Dog, Pig, Cow, Hor, ,

    • 保质期

      按说明书条件可保存1-2年

    • 抗原来源

      见详细说明资料

    • 目录编号

      PL0304506

    • 级别

      超纯

    • 库存

      大量

    • 供应商

      华拓生物

    • 宿主

      Rabbit

    • 应用范围

      WB: 1:100-500 E: 1:500-1000 IP: 1:20-100 IHC: 1:100-500 IF: 1:100-500

    • 浓度

      1mg/ml

    • 靶点

      Phospho-MKK3(Ser218)/MKK6(Ser207)

    • 抗体英文名

      Phospho-MKK3(Ser218)/MKK6(Ser207) Antibody

    • 抗体名

      磷酸化丝裂原活化蛋白激酶MKK3/6抗体

    • 规格

      0.1ml

    产品细节图片1
    Phospho-MKK3(Ser218)/MKK6(Ser207) Antibody
    Cat#:PL0304506
    Isotype:Rabbit Polyclonal IgG
    Immunogen:PhosphoPeptide derived from human MKK3 around the phosphorylation site of Ser218.
    Purification: Immunogen affinity purified

    Storage Buffer: PBS, pH 7.4(1%BSA and 0.1% Sodium azide)

    Background:
    The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis.
    Alternate Names:
    MKK3(Phospho-Ser218); MAP Kinase Kinase 3; Dual specificity mitogen activated protein kinase kinase 3; ERK kinase 3; MAP2K 3; MAPK ERK kinase 3; MAPK kinase 3; MAPKK3; MEK3; Mitogen activated protein kinase kinase 3; MKK3; mMKK3b; PRKMK3; Protein kinase mitogen activated kinase 3; SKK2; zMKK 3; MP2K3_HUMAN; MP2K6_HUMAN.
    Specificity Reaction:Hu, Mo, Rat, Chi, Dog, Pig, Cow, Hor, ,
    Application Notes:WB: 1:100-500 E: 1:500-1000 IP: 1:20-100 IHC: 1:100-500 IF: 1:100-500
    Storage :
    Store at –20ºC for one year. Avoid repeated freeze-thaw cycles. At 4ºC for 2-3 weeks after reconstituted.

    This product for research use only, not for use in humans, therapeutic or diagnostic procedures.
    PLlabs公司可供应相应抗体的生物素、辣根过氧化物酶、荧光素(FITC、PE、 CY3、 CY5、 CY7、 PE- CY3/5/7)标记抗体,欢迎大家咨询订购。

    产品细节图片2 产品细节图片3 产品细节图片4
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    图标文献和实验
    相关实验
    • Using Phospho‐Motif Antibodies to Determine Kinase Substrates

      comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available

    • Signaling Events Initiated by Kappa Opioid Receptor Activation: Quantification and Immunocolocalization Using Phospho-Selective KOR, p38 MAPK, and KIR

      kinase is KIR 3.1 and have developed a phospho-selective antibody against the Y12 motif of this channel. Much like KOR and p38 MAP kinase, phosphorylation of this potassium channel increases following repeated stress. The following chapter discusses

    • 【求助】关于gsk3-beta功能的疑问

      下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。

    图标技术资料

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