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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide derived from human NF-H N-terminus.
- 亚型:
Rabbit Polyclonal IgG
- 形态:
液态
- 保存条件:
保存于-20℃
- 克隆性:
多克隆
- 标记物:
无标记(PLlabs可供应生物素、酶、荧光素标记的抗体)
- 适应物种:
Hu, Mo, Rat
- 保质期:
按说明书条件可保存1-2年
- 抗原来源:
见详细说明资料
- 目录编号:
PL0304310
- 级别:
超纯
- 库存:
大量
- 供应商:
华拓生物
- 宿主:
Rabbit
- 应用范围:
WB: 1:100-500 E: 1:500-1000 IHC: 1:100-500 IF: 1:100-500
- 浓度:
1mg/ml
- 靶点:
NF-H/Neurofilament H/Neurofilament 200
- 抗体英文名:
NF-H/Neurofilament H/Neurofilament 200 Antibody
- 抗体名:
高分子量神经丝蛋白抗体
- 规格:
0.1ml
Storage Buffer: PBS, pH 7.4(1%BSA and 0.1% Sodium azide)
Background:
This product for research use only, not for use in humans, therapeutic or diagnostic procedures.
PLLABS坚持提供高质量的产品和专业的技术服务,并提供完善的技术支持和售后服务。
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文献和实验for this purpose. Grow cells in 200 mls of YPD at 30oC to an OD 600 of 1. Add formaldehyde to the 200 ml culture to a final concentration of 3.7. Fix for 1 hour at room temperature with shaking. Centrifuge cells at 3000 X g. Resuspend
Monoclonal Antibody Production
drip off the end of the tail until you have collected ~200 µl.(You may have to pre-bleed twice,with a week or so between bleeds). (5) Take the collected blood and place at 37 ℃ for 30 min.to remove complement. (6) Place blood at 4 ℃ overnight to clot
Centrifuge cells at 3000 X g. Resuspend in 200 mls Solution A. Repeat wash. Pellet cells as above and resuspend in 4 mls Solution A. Reserve 2 mls of the cell suspension. Spheroplast the remaining 2 ml of the cell suspension by addition
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