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NF-H/Neurofilament H/Neurofila

ment 200 Antibody
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  • ¥2400
  • PLLABS已认证
  • 加拿大
  • PL0304310
  • 2025年07月14日
  • WB: 1:100-500 E: 1:500-1000 IHC: 1:100-500 IF: 1:100-500
  • Rabbit
  • Hu, Mo, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Peptide derived from human NF-H N-terminus.

    • 亚型

      Rabbit Polyclonal IgG

    • 形态

      液态

    • 保存条件

      保存于-20℃

    • 克隆性

      多克隆

    • 标记物

      无标记(PLlabs可供应生物素、酶、荧光素标记的抗体)

    • 适应物种

      Hu, Mo, Rat

    • 保质期

      按说明书条件可保存1-2年

    • 抗原来源

      见详细说明资料

    • 目录编号

      PL0304310

    • 级别

      超纯

    • 库存

      大量

    • 供应商

      华拓生物

    • 宿主

      Rabbit

    • 应用范围

      WB: 1:100-500 E: 1:500-1000 IHC: 1:100-500 IF: 1:100-500

    • 浓度

      1mg/ml

    • 靶点

      NF-H/Neurofilament H/Neurofilament 200

    • 抗体英文名

      NF-H/Neurofilament H/Neurofilament 200 Antibody

    • 抗体名

      高分子量神经丝蛋白抗体

    • 规格

      0.1ml

    产品细节图片1
    NF-H/Neurofilament H/Neurofilament 200 Antibody
    Cat#:PL0304310
    Isotype:Rabbit Polyclonal IgG
    Immunogen:Peptide derived from human NF-H N-terminus.
    Purification: Immunogen affinity purified

    Storage Buffer: PBS, pH 7.4(1%BSA and 0.1% Sodium azide)

    Background:
    Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. When visualised using an electron microscope, neurofilaments appear as 10nm diameter fibres of indeterminate length that generally have fine wispy protrusions from their sides. They are particularly abundant in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. Neurofilaments are composed of a mixture of subunits, which usually includes the three neurofilament triplet proteins neurofilament light (NFL), neurofilament medium (NFM) and neurofilament heavy (NFH). Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.
    Alternate Names:
    Neurofilament 200; 200 kDa neurofilament protein; 200 kD Neurofilament Heavy; NEFH; NEFH; NF200; NF-200; Neurofilament H; Neurofilament heavy polypeptide 200kD; Neurofilament heavy polypeptide 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; Neurofilament triplet H protein; Hypophosphorylated Neurofilament H; NF H; NF-H; NFH; NFH_HUMAN; KIAA0845.
    Specificity Reaction:Hu, Mo, Rat
    Application Notes:WB: 1:100-500 E: 1:500-1000 IHC: 1:100-500 IF: 1:100-500
    Storage :
    Store at –20ºC for one year. Avoid repeated freeze-thaw cycles. At 4ºC for 2-3 weeks after reconstituted.

    This product for research use only, not for use in humans, therapeutic or diagnostic procedures.
    PLlabs公司可供应相应抗体的生物素、辣根过氧化物酶、荧光素(FITC、PE、 CY3、 CY5、 CY7、 PE- CY3/5/7)标记抗体,欢迎大家咨询订购。

    产品细节图片2 产品细节图片3 产品细节图片4
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    图标文献和实验
    相关实验
    • Antibody cleanup

      for this purpose.   Grow cells in 200 mls of YPD at 30oC to an OD 600 of 1. Add formaldehyde to the 200 ml culture to a final concentration of 3.7. Fix for 1 hour at room temperature with shaking. Centrifuge cells at 3000 X g. Resuspend

    • Monoclonal Antibody Production

      drip off the end of the tail until you have collected ~200 µl.(You may have to pre-bleed twice,with a week or so between bleeds). (5) Take the collected blood and place at 37 ℃ for 30 min.to remove complement. (6) Place blood at 4 ℃ overnight to clot

    • Antibody cleanup

      Centrifuge cells at 3000 X g. Resuspend in 200 mls Solution A. Repeat wash. Pellet cells as above and resuspend in 4 mls Solution A. Reserve 2 mls of the cell suspension. Spheroplast the remaining 2 ml of the cell suspension by addition

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