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- 供应商：
西宝生物
- 库存：
大量
- 规格：
250G
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
at 3000g, 4°C in a fixed angle rotor. Make sure caps are screwed on tightly to prevent tubes from collapsing. 8. Pass supernatant through 50 micron Miracloth (Calbiochem #475855, La Jolla, CA) in a funnel into fresh Sarstedt tube. 9. Measure
(for incubations with purified proteins, incubation temperatures may be increased). 3. Add 50 μl of Ca/Mg Solution to the binding reaction at room temperature. 4. Add 1 to 10 μl of DNase I solution (see Hint #1). 5. Mix quickly
(for incubations with purified proteins, incubation temperatures may be increased). 3. Add 50 μl of Ca/Mg Solution to the binding reaction at room temperature. 4. Add 1 to 10 μl of DNase I solution (see Hint #1). 5. Mix quickly
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