Dihydroethidium二氢乙锭,104821-25-

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Dihydroethidium

CAS No. : 104821-25-2

MCE 国际站：Dihydroethidium

产品活性：Dihydroethidium 又称为 DHE，是一种过氧化物指示剂。Dihydroethidium 可穿透细胞膜，形成荧光蛋白复合物，发出蓝色荧光。Dihydroethidium 进入细胞后主要定位于细胞膜、细胞质和细胞核，在细胞核的染色效果最强。Dihydroethidium 产生固有的蓝色荧光，最大激发波长为 370 nm，最大发射波长为 420 nm；Dihydroethidium 脱氢后和 RNA 或 DNA 结合产生红色荧光，最大激发波长为 300 nm，最大发射波长为 610 nm，在实际观察时也可以使用 535 nm 作为激发波长。

研究领域：Others

作用靶点：Fluorescent Dye

In Vitro: General Protocol
Preparation of Dihydroethidium working solution
1.1 Preparation of the stock solution
Dissolve 1 mg of Dihydroethidium in 0.31 mL of DMSO to obtain 10 mM of Dihydroethidium.
Note: It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Dihydroethidium working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of Dihydroethidium working solution.
Note: Please adjust the concentration of Dihydroethidium working solution according to the actual situation.
Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of Dihydroethidium working solution, and then incubate at room temperature for 30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

Storage
-20°C, 1 year
Protect from light

Precautions
1. It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.
2. Please adjust the concentration of Dihydroethidium working solution according to the actual situation.
3. This product is for R&D use only, not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.

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