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文献和实验96 Well Mating Protocol platewise
is between the H1H2 recombination regions which lie just inside two I-SceI cut sites (Sce1-H1-Cassette- 96 well Mating Protocol Preparation: 1. Streak fresh donor plasmid on LB proper antibiotic IPTG/XGAL plate (donor F’ strain contains lac , it will be blue
96 Well Mating Protocol platewise
just inside two I-SceI cut sites (Sce1-H1-Cassette- 96 well Mating Protocol Preparation:1. Streak fresh donor plasmid on LB + proper antibiotic +IPTG/XGAL plate (donor F’ strain contains lac +, it will be blue on the XGAL plate2. Streak recipient plasmid (pMSCV) on LB
96-well RNA In Situ Hybridization Protocol
The RNA in situ procedure described below is based on the protocol developed by Tautz and Pfeifle (Chromosoma 98 (1989), p81), but adapted to allow the screening of 96 RNA probes on whole-mount Drosophila embryos at the same time.Jasprien
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