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- 保存条件:
电询
- 保质期:
电询
- 英文名:
Plasmid Mini Kit II(200)
- 库存:
电询
- 供应商:
上海古朵
- CAS号:
电询
品牌:Omega
Plasmid Mini Kit II(200) (质粒抽提试剂盒)产品特性与优点
安全-无酚氯仿等危险的有机物抽提
简单-硅胶柱过滤操作
快速-在30分钟内完成多个样品的抽提工作
超值-性价比最高
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上海古朵生物提供专业各种试剂盒、质粒抽提试剂盒,培养基、标准品等。严格质控,提供完善的售前、售中、售后服务。上海古朵欢迎您的来电订购!
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文献和实验E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
ethanol (room temperature, 96-100%). Gently mix by inverting tube 6-7 times and incubate at room temperature for1-2 minutes. Plasmid DNA Purification with the HiBind® DNA Mini Column II: 8. Equilibrate the HiBind® DNA column: Add 100ul
E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
gently but throughly by inverting and rotating tube 4-6 times to obtain a cleared lysate. A 2 min incubation at room temperature may be necessary. Avoid vigorous mixing as this will shear chromosomal DNA and lower plasmid purity. (Store Solution II
1. Spin down 1.5 ml of overnight culture in 2ml or 1.5ml tube for 1 minute on high. 2. Asprirate supernatant and resuspend cell pellet in 100 µl Solution I (25 mM Tris-HCl, pH 8.0, 10 mM EDTA). 3. Add 200 µl Solution II (0.2 N NaOH, 1.0% SDS
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