【产品形式】:艾美捷为您提供的His-Tag抗体(克隆号11E12)产品为无色溶液形式,含有100 ul抗体.该产品浓度为1 mg/ml,溶解于0.15 M PBS缓冲液中.请在收到该His-Tag抗体(克隆号11E12)产品后,将其置于-20 °C冷冻保存,建议分装使用以避免反复冻融.
【背景资料】:A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.