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- 详细信息
- 文献和实验
- 技术资料
- 克隆性:
多克隆
- 抗体英文名:
His-Tag Antibody (Clone 11E12) (His-Tag)
- 规格:
100 ul
产品名称:His-Tag抗体(克隆号11E12)|His-Tag Antibody (Clone 11E12) (His-Tag)
【产品形式】:艾美捷为您提供的His-Tag抗体(克隆号11E12)产品为无色溶液形式,含有100 ul抗体.该产品浓度为1 mg/ml,溶解于0.15 M PBS缓冲液中.请在收到该His-Tag抗体(克隆号11E12)产品后,将其置于-20 °C冷冻保存,建议分装使用以避免反复冻融.
【背景资料】:A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
BioVision的公司总部在美丽的旧金山湾地区,它是全球生命科学公司的领导者.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有 卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品,以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代 谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理, 艾美捷与BioVision一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.
点击:His-Tag抗体(克隆号11E12),查看更详细产品说明,更多应用、储存、价格、货期等信息请致电400-6800-868垂询BioVision中国区域授权总代理艾美捷科技有限公司。
关键词:His-Tag,His-Tag抗体(克隆号11E12)
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文献和实验Antibody Screening of Bacteriophage gt11 DNA Expression Libraries
. The oligonucleotides can be used to screen cDNA libraries by hybridization or used as primers to amplify and clone the cDNA by using the polymerase chain reaction.
Antibody Screening of Bacteriophage gt-11 DNA Expression Libraries
. The oligonucleotides can be used to screen cDNA libraries by hybridization or used as primers to amplify and clone the cDNA by using the polymerase chain reaction.
Small scale His-Tag fusion protein purification under denaturative conditions
Small scale His-Tag fusion protein purification under denaturative conditions Introduction High levels of expression of recombinant proteins in a bacterial system can lead to the formation of insoluble aggregates
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