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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 克隆性:
多克隆
- 抗体英文名:
Anti-BrdU antibody (Clone B1G5) (BrdU, BUdR, BrdUrd)
- 规格:
100 ul
产品名称:BrdU抗体(克隆号B1G5)|Anti-BrdU antibody (Clone B1G5) (BrdU, BUdR, BrdUrd)
【产品形式】:艾美捷为您提供的BrdU抗体(克隆号B1G5)产品为无色溶液形式,含有100 ul抗体.该产品浓度为0.5 mg/ml,溶解于PBS缓冲液中.请在收到该BrdU抗体(克隆号B1G5)产品后,将其置于-80°C冷冻保存,建议分装使用以避免反复冻融.
【背景资料】:Bromodeoxyuridine (5-bromo-2’-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analogue of thymidine. The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthesized DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. Antibody specific for BrdU is used to immunocytochemically detect the incorporated BrdU, which is a powerful tool to study cell proliferation, the cytokinetics of normal and neoplastic cells, etc.
BioVision的公司总部在美丽的旧金山湾地区,它是全球生命科学公司的领导者.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有 卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品,以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代 谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理, 艾美捷与BioVision一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.
点击:BrdU抗体(克隆号B1G5),查看更详细产品说明,更多应用、储存、价格、货期等信息请致电400-6800-868垂询BioVision中国区域授权总代理艾美捷科技有限公司。
关键词:BrdU,BrdU抗体(克隆号B1G5)
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- 作者
- 内容
- 询问日期
文献和实验BrdU-Immunoperoxidase Staining of Paraffin Tissue Sections
: Dilute (10x stock, -20ºC) 1/5 in PBS 2.5 N HCl: Dilute concentrated HCl 1/22 (v/v) in H2 O Mouse anti-BrDU: DAKO cat#M0744, $205/mL Biotinylated horse anti-mouse IgG, Avidin, biotin-HRP, Horse serum: Vector, Vectastain ABC kit, #PK4002 $185 Color
Detection of BrdU Incorporation in DNA Synthesizing Cells
Add primary antibody: dilute anti-BrdU monoclonal anitbody (Pharmingen Cat. No. 33281A) in dilution buffer, such that 50 � contains the optimal concentration. Resuspend cell pellet in 50 � of the diluted antibody. Incubate 20 minutes at RT
IMMUNOCYTOCHEMICAL IDENTIFICATION OF PROLIFERATING CELLS IN VASCULAR TISSUE (ANTI-BrdU); Diaminobenzadine (DAB) brown detection Sections of paraformaldehyde fixed, OCT-embedded vascular tissue are sectioned at 7 to 10 mm and stored
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