产品名称:His-Tag标签抗体|His-Tag Antibody,His tag,HHHHHH tag
【产品形式】:艾美捷为您提供的His-Tag抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS, pH 7.2),缓冲液中.请将该His-Tag抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.
【背景资料】:A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.