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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
RIBONUCLEASE A
- 供应商:
西宝生物
- 库存:
10 mg
- 保存条件:
0 C
- 规格:
BIOCHEMICALS
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文献和实验The ribonuclease protection assay (RPA)
The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by the discovery and characterization of DNA-dependant RNA polymerases from the bacteriophages SP6, T
The ribonuclease protection assay (RPA) is a sensitive technique for the analysis of total cellular RNA. It involves generating a specific antisense riboprobe, hybridizing the probe to total RNA, removing unprotected RNA by RNases, and finally
(Mg2+ , Mn2+ ), an RNA sample is most likely to be rapidly degraded by a contaminating ribonuclease (RNase). RNases are difficult to completely remove or inactivate during RNA isolation procedures, and they may be introduced into the sample
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