PHOSPHODIESTERASE

Assessment of Phosphodiesterase Isozyme Contribution in Cell and Tissue Extracts

Cyclic nucleotide phosphodiesterases (PDEs), which are ubiquitously distributed in mammalian tissues, play a major role in cell signaling by hydrolyzing cyclic adenosine 3′,5′-monophosphate and cyclic guanosine 3′,5′-monophosphate. Owing

The Analysis and Assay of Cyclic Nucleotide Phosphodiesterase Isoenzyme Activity

are inactivated by a large group of enzymes collectively known as 3′,5′-cyclic nucleotide phosphodiesterases (PDEs; EC 3.1.4.17), which catalyze the hydrolytic cleavage of the 3′ phosphodiesterase bond to form the corresponding 5′ nucleotide (i.e., 5′AMP or 5′GMP

磷酸二酯酶 phosphodiesterase

为磷酸酯酶之一。可催化正磷酸的二酯水解生成磷酸单酯和醇的酶。一般与磷酸单酯酶同时存在，也存在于蛇毒、血清（最适pH8.5— 9.0）、米糠、肝脏和高淀粉酶（ Tak－ diastase）（最适 pH5.5）等，多核苷酸酶和水解胆碱的甘油磷酸酯的溶血磷酯酶也是一种磷酸二酯酶。（参见脾核酸外切酶、蛇毒磷酸二酯酶）。