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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100 g
- 保存条件：
ROOM TEMPERATURE
- 英文名：
MALT EXTRACT
- 供应商：
西宝生物
- 规格：
BIOCHEMICALS
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文献和实验Preparation of nuclear extract and cytoplasmic extract
PMSFBuffer D (Dialysis Buffer): For 2 L20 mM HEPES,pH 7.9 1 M HEPES 7.9 40 ml20 % Glycerol Glycerol 400 ml100 mM KCl3 M KCl66.67 ml0.2 mM EDTA 0.5 M EDTA 800 µl0.5 mM DTT 1M DTT 1 ml0.2 mM PMSF 0.2 M PMSF 2 mlSplicing Extract Preparation1.Resuspend cell
us to succesfully immunodeplete and characterize three different proteins: a) During extract prep, after washing out cysteine with XB, wash eggs 2-3x with CSF-XB (no PIs) before washing with CSF-XB + PIs and then process as usual. b) Crush the eggs at 10
+ PIs. Add 150 ul of clarified extract to each tube. Rotate for 1 hr at 4 deg.C ensuring that beads are mixing well. Pellet and transfer supe to a different tube. Aliquot and freeze 20 ul aliquots in green tubes (XKCM1 deplete
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