【背景资料】:5-FAM-X, SE contains the aminohexanoyl spacer (known as 慩) between the FAM fluorophore and the succinimidyl ester. The 慩 spacer separates the fluorophore from the biomolecule to which it is conjugated. The 慩 spacer may potentially reduce the quenching that typically occurs when a fluorescein molecule is conjugated to proteins or other biopolymers (such as nucleic acids).