RHODAMINE-B-ISOTHIOCYANATE

Nystatin-Rhodamine B Assay for Estimating Activity of Killer Toxin from Kluyveromyces lactis

Killer toxin activity is commonly determined by the well test (1 ), which ensures the inhibition zone on a substratum of sensitive strain cells. The Rhodamine B assay described in Chapter 31 is based on estimation of the fraction of killed

Rhodamine B Assay for Estimating Activity of Killer Toxins Permeabilizing Cytoplasmic Membranes

Killer yeasts secrete proteins (killer toxins, zymocins) that kill sensitive yeast strains (1 ,2 ). The killer strain is immune (R+ ) to the effects or its own toxin, but it can be sensitive to the toxins of another immunity group

Conjugation of Antibodies to Fluorescein or Rhodamine

The isothiocyanate derivative of the fluorophore, fluorescein or rhodamine, is coupled to the amino groups of IgG antibody in a one-step procedure and excess label is removed by gel filtration.