V.G染色液

Multiplex PCR for Detection of the Prothrombin 3-UTR (G20210A) Polymorphism and the Factor V Leiden Mutation

disorder increases. A recently described G→A polymorphism at position 20210 in the 3′-untranslated region of the prothrombin gene (F2 3′-UTR) has been found to be associated with an increased risk of venous thrombotic disease. In the Leiden Thrombophilia

Detection of Factor V Leiden and Prothrombin c.20210G>A Allele by Roche Diagnostics LightCycler

Venous thrombosis affects one in one thousand people each year, and in many countries, it is a major cause of morbidity and death in hospitalised patients. Factor V Leiden and the prothrombin c.20210G>A transition are relatively common

Annexin V 荧光双染细胞凋亡检测试剂盒实验操作指南

Annexin V是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸PS有高度亲和力，可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合，将Annexin V标记上荧光染料利用流式细胞仪或荧光显微镜可检测细胞凋亡。 由于凋亡晚期或坏死细胞膜完整性丧失，细胞核染色液可进入细胞内染色细胞核，搭配Annexin V使用可将处于不同凋亡时期的细胞区分开。 A．悬浮细胞： 按照实验方案进行凋亡诱导，300 g离心5 min，弃上清，收集细胞，用PBS洗涤细胞一次，轻轻重悬浮细胞并计数。 取1~5 ×