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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
单克隆
- 抗体英文名：
Anti-SPY (spindly)
- 抗体名：
抗spindly抗体
抗spindly抗体产品介绍
The SPINDLY (SPY) protein of Arabidopsis is a negative regulator of gibberellin (GA) response. The SPY protein has 10 copies of the tetratricopeptide repeat (TPR) at the N terminus. TPR motifs function as protein-protein interaction domains. Several spy alleles are affected only in the TPR region suggesting that protein-protein interactions mediated by this domain are important for proper GA signaling. We have used a reverse genetics approach to further investigate the role of the TPR domain.
牋牋The TPR domain of SPY was overexpressed in wild-type, gai, and spy plants. Expression of the TPR domain alone is not sufficient to rescue spy mutants. Expression of the TPR domain in a wild-type background produces phenotypes similar to those caused by loss-of-function spy mutants including resistance to GA biosynthesis inhibitors, short hypocotyl length, and early flowering. The dwarfing of the floral shoot internodes caused by the gai mutation was suppressed by expression of the TRP domain. Expression of the TPR domain had no effect on the abundance of endogenous SPY mRNA. The TPR domain was found to interact with SPY both in vitro and in yeast two-hybrid assays. These data indicate that the TPR domain of SPY can participate in protein-protein interactions and that these interactions are important for the proper functioning of SPY
xy-0358xy-xyy3 Donkey Anti-Guinea pig IgG/Cy3Cy3标记的驴抗豚鼠IgG
xy-0358xy-AF488 Donkey Anti-Guinea pig IgG/Alexa Fluor 488Alexa Fluor 488标记的驴抗豚鼠IgG
xy-0358xy-xyy5.5 Donkey Anti-Guinea pig IgG/Cy5.5Cy5.5标记的驴抗豚鼠IgG
xy-0358xy-xyE-xyY5 Donkey Anti-Guinea pig IgG/PE-CY5PE-CY5标记的驴抗豚鼠IgG
xy-0358xy-Axyxy Donkey Anti-Guinea pig IgG/APCAPC标记的驴抗豚鼠IgG
xy-0358xy-Axy Donkey Anti-Guinea pig IgG/AP碱性磷酸酶（AP）标记的驴抗豚鼠IgG
xy-0358xy-FITxy Donkey Anti-Guinea pig IgG/FITCFITC标记的驴抗豚鼠IgG
xy-0358xy-RBITxy Donkey Anti-Guinea pig IgG/RBITC罗丹明标记的驴抗豚鼠IgG
xym-0297M-RBITxy Mouse Anti-human IgG(3D3)/RBITC罗丹明标记的鼠抗人IgG单克隆抗体
xym-0297M-Golxy Mouse Anti-human IgG(3D3)/Gold胶体金标记的鼠抗人IgG单克隆抗体
xym-0297M-FITxy Mouse Anti-human IgG(3D3)/FITCFITC标记的鼠抗人IgG单克隆抗体
xym-0297M-Axy Mouse Anti-human IgG(3D3)/AP碱性磷酸酶（AP）标记的鼠抗人IgG单克隆抗体
xym-0297M-xyE-xyy3 Mouse Anti-human IgG(3D3)/PE-Cy3PE-Cy3标记的鼠抗人IgG单克隆抗体
xym-0297M-xyE-xyY5 Mouse Anti-human IgG(3D3)/PE-CY5PE-CY5标记的鼠抗人IgG单克隆抗体
xym-0297M-Axyxy Mouse Anti-human IgG(3D3)/APCAPC标记的鼠抗人IgG单克隆抗体
xym-0297M-AF350 Mouse Anti-human IgG(3D3)/Alexa Fluor 350Alexa Fluor 350标记的鼠抗人IgG单克隆抗体
xym-0297M-AF488 Mouse Anti-human IgG(3D3)/Alexa Fluor 488Alexa Fluor 488标记的鼠抗人IgG单克隆抗体
xym-0297M-AF555 Mouse Anti-human IgG(3D3)/Alexa Fluor 555Alexa Fluor 555标记的鼠抗人IgG单克隆抗体
xym-0297M-AF647 Mouse Anti-human IgG(3D3)/Alexa Fluor 647Alexa Fluor 647标记的鼠抗人IgG单克隆抗体
xym-0297M-xyE-xyy5.5 Mouse Anti-human IgG(3D3)/PE-Cy5.5PE-Cy5.5标记的鼠抗人IgG单克隆抗体
xym-0297M-xyE-xyy7 Mouse Anti-human IgG(3D3)/PE-Cy7PE-Cy7标记的鼠抗人IgG单克隆抗体
xym-0297M-xyy5.5 Mouse Anti-human IgG(3D3)/Cy5.5Cy5.5标记的鼠抗人IgG单克隆抗体
xym-0297M-xyy7 Mouse Anti-human IgG(3D3)/Cy7Cy7标记的鼠抗人IgG单克隆抗体
xym-0297M-xyy3 Mouse Anti-human IgG(3D3)/Cy3Cy3标记的鼠抗人IgG单克隆抗体
xym-0297M-xyy5 Mouse Anti-human IgG(3D3)/Cy5Cy5标记的鼠抗人IgG单克隆抗体
抗spindly抗体
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文献和实验有了质量好的抗原，还必须选择适当的免疫途径，才能产生质量好（特异性强和效价高）的抗体。 （一）用于免疫的动物 作免疫用的动物有哺乳类和禽类，主要为羊、马、家兔、猴、猪、豚鼠、鸡等，实验室常用者为家兔、山羊和豚鼠等。动物种类的选择主要根据抗原的生物学特性和所要获得抗血清数量，如一般制备抗r-免疫球蛋白抗血清，多用家兔和山羊，因动物反应良好，而且能够提供足够数量的血清，用于免疫的动物应适龄
抗酶抗体的优点，在于酶和抗体结合时，不需要通过化学交联剂交联，抗体的失活少。在酶复合物形成后，同时就具有酶的活性，遇到相应的底物即呈现催化显色作用。必须注意，抗酶抗体必须用与第一抗体同种来源的动物制备。 于家兔的两个后足掌内各注入弗氏完全佐剂 ( 含活卡介苗 12mg/ml) 和过氧化物 (5mg/ml) 的等量混合乳化液 0.5ml ，两星期后，于两侧腘淋巴结内注入同样液体。一周后静脉注射 0.2ml~0.4ml ，过氧化物酶液 ( 同样浓度
（一）抗原制备 Ig是免疫球蛋白，对异种动物而言，是良好的抗原物质。可以刺激异种动物产生抗Ig血清，经适当提取后，产生抗Ig抗体，又叫第二抗体或抗抗体。在多数的间接标记反应中，均需制备抗Ig抗体。 （二）材料与 试剂 1．提取的动物Ig 2．弗氏完全佐剂和弗氏不完全佐剂 3．青霉素和链霉素 4．实验动物 兔 5．其它材料及 试剂
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