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- 详细信息
- 文献和实验
- 技术资料
- 克隆性:
单克隆
- 抗体英文名:
Anti-IFN-β (Interfer
- 抗体名:
干扰素-β抗体(大鼠、小鼠)
The mammalian type I Inteferons (IFN1) are produced in response to viral infection and other inducers. They are divided into alpha and beta subtypes leukocytes and fibroblasts reactivity. The human IFN alphas are encoded by a family of at least 15 different genes, while IFN beta is the unique member of its subtype. There is approximately 50% amino acid homology between the alpha and beta subtypes. Both IFN subtypes are pleiotropic cytokines and have a similar range of biological activities. Differences between alpha subtypes, and between IFN alpha and betas, are in potency and cell type specific activities. In particular, IFN beta elicits a markedly higher antiproliferation response in some cell types such as, embryonal carcinoma, melanoma and melanocytes than do IFN alphas. Higher potency of IFN beta in treatment of multiple sclerosis and certain cancers has been observed. Type I IFNs signal through binding to a common cell surface receptor. Two chains of the receptor, IFNAR1 and IFNAR2, have been identified. Both chains are necessary for function and in the absence of either there is neither high affinity binding nor biological activity. The intracellular portions of the receptor subunits are bound by tyrosine kinases, Jak1 and Tyk2, members of the Janus kinase family. Upon ligand binding these kinases are activated and phosphorylate members of the STAT family of transcription factors, as well as IFNAR1 and 2.
Also known as:
Fibroblast interferon; IFB; IFF; IFN beta; IFNB 1; IFNB ; IFNB1; Interferon beta 1 fibroblast ; Interferon beta precursor; MGC96956
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干扰素-β抗体(大鼠、小鼠)
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文献和实验时期 胎龄(日) 胚胎发育 小鼠 大鼠 1 1 1 单核细胞(输卵管内) 2 2 2 细胞期(输卵管内) 3 2 3.25 4 细胞期(输卵管内) 4 2.5 3.5 8-12 细胞期(输卵管内) 5 3 3. 桑椹期(输卵管—子宫上部) 6 3.5 4 囊胚初期(子宫内) 7 4 5 囊胚期 8 4.5 6 植入开始 9 5 6.75 内外胚层分化 10 5.5 7.25 植入
可信的动物实验结果不仅依赖于科学的实验设计,也依赖于标准化的实验动物饲养。饲养小鼠、大鼠必须使用垫料是因为啮齿动物更喜爱平整带垫料的笼底,而不是金属网笼底。垫料可以吸收尿液和粪便,方便清洁。对小鼠、大鼠来说,与垫料直接接触增加了它们表达天性的机会,如搜寻、挖掘、和筑巢。 现在国内很容易获得商品化合格的实验动物,但是由于质量、价格等原因,研究机构往往不容易获得合适的垫料。我国《实验动物环境及设施》标准(GBl4925-2010)规定:垫料的材质应符合动物健康和福利要求,应满足吸湿性好、尘埃
实验板上。如若不麻醉,则将小鼠放人保定架里,固定好保定架的封口。 2、大鼠的捉拿、保定 大鼠的捉拿有一些危险性,因大鼠受攻击时,会咬人抓人,尽量不用突然猛抓的办法。捉拿大鼠特别注意不能捉提尾尖,也不能让大鼠悬在空中时间过长,否则易激怒大鼠和易致尾部皮肤脱落。抓大鼠时若操作者不熟练,或者大鼠特别凶猛,操作者最好戴防护手套(帆布或硬皮质均可)。如若是灌胃、腹腔注射、肌肉和皮下注射时,可采用与小鼠相同的手法,即用拇、食指捏住鼠耳朵头颈部皮肤,余下三指紧捏住背部皮肤,置于掌心中,调整大鼠
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