The antibody was purified by affinity chromatography, and conjugated with Biotin under optimal conditions. The solution is free of unconjugated Biotin.
Recommended Usage
Each lot of this antibody is quality control tested by ELISA analysis. For ELISA detection, the suggested use of this reagent is 1:1000 dilution. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
ELISA Detection
Application Notes
The biotinylated poly5019 antibody is useful as the detection antibody in a sandwich ELISA, when used in conjunction with the purified capture antibody (cat. # 507802 or 507808).
Description
Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The poly5109 antibody reacts with rat interferon-gamma (IFN-γ).
Storage
The antibody solution should be stored undiluted at 4°C.