Human; This antibody does not cross react with mouse IL-33.
Immunogen
Mature recombinant protein human Interleukin-33 (Ser112-Thr270)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting applications, a concentration of 1 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
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Applications
WB - Quality tested
Application Notes
The optimal concentration should be determined by titration for each individual assay of interest.
Description
IL-33, also known as NFHEV and DVS 27, is a 30 kD proinflammatory protein that belongs to IL-1 superfamily. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL1α or IL1β stimulation. IL-33 interacts with IL1RL1/ST2 receptor and IL-1 receptor accessory protein (IL1RAP) in order to activate intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. Recombinant IL-33 has been shown to enhance Th2-associated immune responses and potently increase mast cell proliferation and cytokine production.