Peptide mapping to the carboxy terminal domain of human SIRT1.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.
Preparation
The antibody was purified by affinity chromatography.
Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use10µl per 5 ml antibody dilution buffer for each mini-gel.It is recommended that the reagent be titrated for optimal performance for each application.
Applications
WB-Quality tested IP - Reported in the literature
Description
SIRT1, the human homolog of the S. cerevisiae Sir2 protein, functions as an NAD-dependent deacetylase of a number of nonhistone substrates including p53. In response to DNA damage, SIRT1 binds and deacetylates the p53 protein at c-terminal Lys382 residue and attenuate p53-mediated functions. When overexpressed in mouse embryo fibroblasts, SIRT1 antagonizes PML-induced acetylation of p53 and rescues PML-mediated premature cellular senescence. In mammalian cells, SIRT1 appears to control the cellular response to stress by regulating the FOXO family of forkhead transcription factors. The Poly6343 antibody has been shown to be useful for western blotting and immunoprecipitation of human SIRT1 protein.
Storage
The antibody solution should be stored undiluted at 4°C. DO NOT FREEZE..