This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by antigen-affinity chromatography.
Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
WB - Quality tested
Description
p63 (TA) is a member of the p53 family. This protein has multiple isoforms TA p63-α, -β, -γ, and δN p63-α, -β, -γ. The molecular weight of the TA p63 isoforms α=77 kD, β=62 kD, and γ=56 kD. This nuclear protein is essential for limb formation, epidermal morphogenesis, epithelial stem cell regeneration; δN isoforms are antagonistic to p53; TA isoforms can transactivate p53 targets. The p63 δN isoforms repress TA isoforms; mutant p53 abrogates p63 transactivation This protein interacts with p53, p73, and the various p63 TA- and δN isoforms. The Poly6189 antibody recognizes human p63 TA isoforms and has been shown to be useful for Western blotting.