手机验证
蓝冰
多克隆
1 mg
Clone | MP6-XT22 |
Isotype | Rat IgG1, κ |
Reactivity | Mouse |
Immunogen | E. coli-expressed, recombinant mouse TNF-α |
Formulation | 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test. |
Preparation | The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography. |
Concentration | 1mg/ml |
Recommended Usage | Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse TNF-α recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application. |
Applications | ELISA, ICFC - Quality tested |
Application Notes | ELISA or ELISPOT Detection: The biotinylated MP6-XT22 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 6B8 antibody (Cat. No. 510802/510804) as the capture antibody. |
Application References | 1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (Neut) |
Description | TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is a 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. |
Storage | The antibody solution should be stored undiluted at 4°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions. |
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