Clone |
C11.5 |
Isotype |
Mouse IgG1, κ |
Reactivity |
Human, IL-12/IL-23 p40 subunit (monomer, homodimer and heterodimer IL-12 p35/p40 or IL-23 p19/p40) |
Immunogen |
CHO-expressed, recombinant human IL-12 p70 heterod |
Formulation |
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w/v) BSA (USA origin). |
Preparation |
The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488. |
Recommended Usage |
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents. |
Applications |
ICFC - Quality tested |
Application Notes |
Flow Cytometry3,5: The fluorochrome-labeled C11.5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-12 p40 (monomeric, heterodimeric, homodimeric) -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Neutralization6: The LEAF™ purified antibody (Endotoxin Additional reported applications (for the relevant formats) include: Western blotting,ELISA Capture1,2, ICFC Blocking, immunohistochemical staining4 of paraformaldehyde-fixed, saponin-treated tissue frozen sections, and immunocytochemistry. Note: For testing human IL-12/IL-23 p40 (monomer, dimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430701 to 430706) are specially developed and recommended. For testing human IL-12 p70 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431701 to 431706) are specially developed and recommended. |
Application References |
1. D'Andrea A, et al. 1992. J. Exp. Med. 176:1387. 2. D'Andrea A, et al. 1993. J. Exp. Med. 178:1041. 3. Prussin C, et al. 1995. J. Immunol. Meth. 188:117. 4. Leifeld L, et al. 2002. Hepatology 36:1001. 5. Pekkari K, et al. 2001. Blood 97:3184. 6. Bolovan-Fritts CA, et al. 2004. J. Virol. 78:13173. |
Description |
The C11.5 antibody reacts with human IL-12 p40 subunit of the IL-12 p70 heterodimer and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer. The C11.5 antibody can neutralize the bioactivity of natural or recombinant IL-12 p70. |
Storage |
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |