Clone |
MQ1-17H12 |
Isotype |
Rat IgG2a, κ |
Reactivity |
Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Sooty Mangabey |
Immunogen |
E. coli - expressed recombinant human IL-2 |
Formulation |
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA). µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
Preparation |
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC. |
Concentration |
test size: lot-specific; µg size: 0.5 mg/ml |
Recommended Usage |
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change. " |
Applications |
ICFC - Quality tested |
Application Notes |
ELISA or ELISPOT Capture2,3: The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5176 antibody (Cat. No. 517601) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. Neutralization2,3: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IL-2 bioactivity (Cat. No. 500313). The MQ1-17H12 antibody can neutralize the bioactivity of natural or recombinant IL-2. Additional reported applications (for the relevant formats) include: immunoprecipitation2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections1,4-6,8, and immunocytochemistry. Note: For testing human IL-2 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431801 to 431806) are specially developed and recommended. |
Application References |
1. Andersson J, et al. 1994. Immunology 83:16. (IHC) 2. Abrams J, et al. 1992. Immunol. Rev. 127:5. (IP) 3. Abrams JS. 1995. Curr. Prot. Immunol. Unit 6.20. 4. Fernandez V, et al. 1994. Eur. J. Immunol. 24:1808. (IHC) 5. Skansen-Saphir U, et al. 1994. Eur. J. Immunol. 24:916. (IHC) 6. Andersson U, et al. Detection and Quantification of Gene Expression. New York:Springer-Verlag. (IHC) 7. Prussin C, et al. 1995. J. Immunol. Methods. 188:117. 8. Raqib R, et al. 2002. Infect. Immun. 70:3199. (IHC) 9. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed 10. Colleton BA, et al. 2009. J Virol. 83:6288. PubMed 11. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 12. Rout N, et al. 2010. PLoS One 5:e9787. (FC) " |
Description |
IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes. |
Storage |
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |