The antibody was purified by affinity chromatography, and conjugated with Cy3 under optimal conditions. The solution is free of unconjugated Cy3.
Concentration
0.5
Concentration Units
mg/ml
Recommended Usage
It is recommended that the reagent be titrated for optimal performance for each application.
Applications
IF
Application Notes
This conjugated goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.
Application References
1. Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed 2. Tokarev AA, et al. 2011. J. Virol. 85:51. (IF) PubMed 3. Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed
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Description
Goat anti-rat IgG antibody reacts with the heavy chains of rat IgG and the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.