The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Applications
FC - Quality tested
Application References
1. Nagata K, et al. 1999. J. Immunol. 162:1278. (FC) 2. De Fanis U, et al. 2007. Blood 109:4343. (FC) 3. Tsuda H, et al. 2001. Clin. Exp. Immunol. 123:105. (FC) 4. Hamada K, et al. 2004. Allergology International 53:179. (FC)
Description
CD294, also known as CRTH2, is a seven-transmembrane protein coupled with heterotrimeric G proteins. CRTH2 is the prostaglandin D2 receptor and is expressed by Th2 cells, eosinophils, and basophils. CD294 prevents the apoptosis of Th2 cells and mediates the chemotaxis of CRTH2 expressing cells to the sites of allergic inflammation, such as the asthmatic lung.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.