The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
FC - Quality tested
Application Notes
Clone S5/1 blocks the binding of C5a to CD88.
Application References
1. Kiener HP, et al. 1998. Arthritis Rheum. 41:233. 2. Elsner J, et al. 1994. Blood 83:3324. 3. Oppermann M, et al. 1993. J. Immunol. 151:3785.
Description
CD88 is a single chain protein with seven membrane-spanning regions and has a MW of 43 kD. CD88 is expressed by monocytes, neutrophils and eosinophils. Also, there have been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes and vascular endothelial cells. CD88 is coupled to heterotrimeric G proteins such as Gi, and after C5a binding, the signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules, resulting in chemotaxis, granule enzyme release and superoxide anion production.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.