The antibody was purified by affinity chromatography.
Concentration
0.5mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
FC - Quality tested IP - Reported in the literature
Application Notes
Clone GHI/61 binds to domain 7 of CD163. Additional reported applications (for the relevant formats) include: immunoprecipitation1.
Application References
1. Pulford K, et al. 1992. Immunology 75:588. (IP) 2. Law SK, et al. 1993. Eur. J. Immunol. 23:2320. 3. Madsen M, et al. 2004. J. Biol. Chem. 279:51561. 4. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
Description
CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, Hemoglobin-Haptoglobin Complex Receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kuffer cells, monocytes, subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP) resulting in soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammation diseases.
Storage
The antibody solution should be stored undiluted at 4°C.