The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
0.2mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
FC - Quality tested
Application References
1. Lefrancois L, et al. 1990. Cell. 66:33 2. Sperling AI, et al. 1992. J. Immunol. 149(10):3200 3. Pereira P, et al. 1997. Proc. Natl. Acad. Sci. USA. 94:5761
Description
Lymphocytes expressing γ/δ antigen receptors constitute a minor population in lymphoid tissue, but a major population within epidermal, gastraintestine, and vaginouterine epithelia. Gamma/Delta T cells are involved in immunological surveillance of epithelia separating the external environment from the internal milieu. The GL2 antibody reacts with Vδ4 T-cell receptor (TCR) bearing T cells, which are the predominant γ/δ T cells in intestinal epithelium (γ/δ IEL). Gamma/delta T cells has been found in mammary glad as well. The frequency of Vδ4 TCR-bearing cells varies among inbred strains of mice.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.