The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
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Applications
FC - Quality tested
Application Notes
The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining.
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Application References
1. Ohl L, et al. 2004. Immunity 21:279. 2. Ritter U, et al. 2004. J. Leukocyte Biol. 76:472. 3. Lan YY, et al. 2005. Am. J. Transplant. 5:2649. (FC) 4. Lee JH, et al. 2007. J. Immunol. 178:301. (FC) PubMed 5. Kurooka M and Kaneda Y. 2007. Cancer Res. 67:227. (FC) PubMed 6. Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) 7. Sakai N, et al. 2006. P. Natl. Acad. Sci. USA 103:14098. (FC) 8. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) 9. Hwang IY, et al. 2007. J. Immunol. 179:439. (FC) PubMed 10. Kang SG, et al. 2007. J. Immunol. 179:3724. 11. Mao A et al. 2005. J. Immunol. 175:5146. PubMed 12. Allende ML, et al. 2008. FASEB J. 22:307. PubMed 13. Kang SG, et al. 2007. J. Immunol. 179:3724. PubMed 14. Chen H, et al. 2005. J. Immunol. 175:591. PubMed 15. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
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Description
CD197 is also known as C-C chemokine receptor 7 (CCR7) or EBI-1. CCR7 is a G-coupled rhodopsin-like member of the GPCR superfamily with a predicted molecular weight of 43 kD that is expressed on hematopoietic stem cells, most naive T cells, some memory T cells, B subset, and mature dendritic cells. CCR7 is a receptor for the chemokines CCL19 (MIP3 beta) and SLC (6CKine, Exodus-2, TCA-4, CCL21) that plays a role in thymocytes development, T cell adhesion at intestinal sites, the homeostatic recirculation of memory T cells, and chemotaxis.
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Storage
The antibody solution should be stored undiluted at 4°C. Do not freeze.