The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration
0.2mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and a maximum emission of 690 nm.
Applications
FC - Quality tested
Application Notes
The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.
Application References
1. Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP) 2. Sobel ES, et al. 1993. J. Immunol. 150:673. 3. Karlhofer FM, et al. 1991. J. Immunol. 146:3662. 4. Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC) 5. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC) 6. Lawson BR, et al. 2007. J. Immunol. 178:5366. 7. Lee JW, et al. 2006. Nature Immunol. 8:181. 8. Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed 9. Jordan JM, et al. 2008. 76:3717. PubMed 10. Kenna TJ, et al. 2008. Blood111:2091. PubMed
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Description
CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3 and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.