FliC is expressed in motile bacteria including Salmonella and E. Coli. The epitope recognized by this antibody is not known. There is a possibility that some flagellins are not recognized by this FLIC-1 antibody.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
WB - Quality tested IF, IP - Reported in the literature
Application References
1. Uchiya K, et al. 2008. Microbiology. 154:3491. (IF) PubMed 2. Huang LY, et al. 2009. J. Clin Microbiol. (IP)PubMed 3. Ibarra JA, et al. 2010. Microbiology. 156:1120. PubMed 4. Crawford RW, et al. 2010. J. Bacteriol. 192:2981. (WB) PubMed 5. Knodler LA, et al. 2010. Proc Natl Acad Sci USA. 107:17733. PubMed 6. Kajikawa, A., et al. 2011. Appl Environ Microbiol. 77:6587. PubMed. 7. Eom JS, et al. 2012. J. Bacteriol. 194:4332. PubMed.
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Description
FliC is a protein expressed in many motile enteric bacteria including Salmonella and Escherichia. The C- and N-terminal regions of the protein among various strains of bacteria are well-conserved. However, there is great variability of length and amino acid sequence in the central region. For example, E. coli flagellin have been reported to vary in size from 36 K to 69 K in MW. The epitope recognized by this FLIC-1 monoclonal antibody is not known. FliC is a subunit protein that polymerizes (in conjunction with other proteins) to form the filaments of bacterial flagella in a precise order. Flagellin is a potent ligand of toll-like receptor 5 (TLR5). By binding to TLR5, flagellin induces activation of NF-kB and triggers the production of cytokines and innate immune responses.
Storage
The antibody solution should be stored undiluted at 4°C.