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Recombinant Human IL-27 (carrier-free)参数介绍>>>>
Product Type
Recombinant Proteins
Derivation
The human IL-27 consists of two subunits linked via a 4x(GGGS) bridge: Human CD33 signal peptide (Met1-Ala16) human EBV-3 induced gene 3 (EBI3) (Accession# NM_005755 Arg21 – Lis229) and human p28 (Accession# NM_145659 Phe29 - Pro243) 10 His-tag. Mouse I
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 20 mM NaHPO4, pH 6, 0.60 M NaCl.
Preparation
10-100 µg sizes are bottled at 100 µg/mL. 500 µg and larger sizes are bottled at the concentration indicated on the vial.
Concentration
10 and 25 µg sizes are bottled at 100 µg/mL.
Activity
ED50 = 60 - 90 ng/ml, corresponding to a specific activity of 1.1 - 1.7 x 104 units/mg, as determined by mouse splenocytes IL-2 inhibition induced by hIL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies.
Function
IL-27 triggers rapid clonal expansion of antigen-specific naive human and mouse CD4 T cell. IL-27 promotes the early Th1 differentiation through up-regulation of ICAM-1 and T-bet, and suppresses the differentiation to Th2 and Th17. In addition, IL-27 suppresses the cytokine production by activated CD4+ T cells in vitro, and TNF-α and IL-12 production by macrophages. This suppressive mechanism is considered to be mediated through IL-10. IL-27 plays a dominant function together with TGF-β in generating IL-10-producing anti-inflammatory Tr1 cells. Additionally, IL-27 exerts IFN-like functions in liver cells, and is a negative regulator of human neutrophils function. IL-27 is predominantly produced early after stimulation of APCs by TLR ligands or infectious agents, and it is induced by TNF-α in human macrophages.
Applications
Bioassay
Description
IL-27 is a heterodimeric cytokine that consists of EBI3, an IL-12p40-related protein, and p28, an IL-12p35-related polypeptide. IL-27 can be allocated to the IL-6/IL-12 superfamily of cytokines which also includes IL-23. This family is defined based on similarities in the structural motifs of the ligands, such as a common four-helix bundle and their receptors, which contain a hematopoietin receptor domain. IL-27 signaling occurs via a receptor complex composed of the signal transducing receptor chains WSX-1 and glycoprotein (gp) 130. Whereas WSX-1 is the IL-27–specific receptor chain, gp130 is the common receptor subunit of IL-6 type cytokines. IL-27 is expressed at sites of inflammation in cytokine-driven autoimmune/inflammatory diseases, such as rheumatoid arthritis, psoriasis, inflammatory bowel disease, and sarcoidosis.
Storage
Unopened vial can be stored at -20°C for six months or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. After dilution, the cytokine can be stored at 4°C for one month or from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.