The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
0.2mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining by flow cytometry, the suggested use of this product is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
ICFC - Quality tested
Application Notes
ELISA or ELISPOT Capture1: The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1. The LEAF™ purified antibody is suggested for ELISPOT capture. Flow Cytometry2: The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Neutralization1,4,6: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of mouse/rat MCP-1 bioactivity in vivo and in vitro (Cat. No. 505906). Additional reported applications (for the relevant formats) include: Western blotting3, immunohistochemistry5 of paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Application References
1. Luo, Y., et al. 1994. J. Immunol. 153:3708. 2. Zhang, Y., et al. 2002. J. Immunol. 168:3088. 3. Luo, Y., et al. 1999 J. Immunol. 163:3985. 4. Morrison, B. E., et al. 2003 J. Clin. Invest. 112:1862. 5. Hancock, W. W., et al. 1997 Transplantation 64:696. 6. Yu, R., et al. 2006 Obesity 14:1353.
Description
Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 2H5 antibody reacts with mouse, rat, and human MCP-1. The 2H5 antibody can neutralize the bioactivity of natural or recombinant MCP-1.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.