手机验证
蓝冰
多克隆
25 ug
Clone | XMG1.2 |
Isotype | Rat IgG1, κ |
Reactivity | Mouse |
Immunogen | E. coli -expressed, recombinant mouse IFN-γ |
Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
Preparation | The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody. |
Concentration | 0.2mg/ml |
Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
Applications | ICFC - Quality tested |
Application Notes | ELISA1-4,11,14 or ELISPOT5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. |
Application References | 1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut) |
Description | Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. |
Storage | The IFN-γ antibody solution should be stored undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze. |
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