The antibody was purified by affinity chromatography.
Concentration
0.5mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
ELISA Capture-Quality tested IHC - Reported in the literature
Application Notes
ELISA or ELISPOT Capture1,2: The purified BVD13-3A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD11-37G10 antibody (Cat. No. 506702) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Application References
1. Abrams J. 1995. Curr. Prot. Immunol.. John Wiley and Sons New York. Unit 6.20. 2. Abrams J, et al. 1992. Immunol. Rev. 127:5. 3. Andersson J, et al. 1994. Immunology 83:16. 4. Sander B, et al. 1991. Immunol. Rev. 119:65. 5. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
Description
Granulocyte - colony stimulating factor (G-CSF) is a potent stimulator of bone marrow cells especially those of neutrophilic granulocyte lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. G-CSF is produced primarily by monocytes and macrophages upon activation by endotoxin, TNF-alpha or IFN-gamma. The BVD13-3A5 antibody reacts with human granulocyte-colony stimulating factor (G-CSF). The BVD13-3A5 antibody can neutralize the bioactivity of natural or recombinant G-CSF.
Storage
The antibody solution should be stored undiluted at 4°C.