The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
0.5
Concentration Units
mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
FC - Quality tested
Application Notes
This FITC conjugated polyclonal goat anti-mouse IgG antibody is useful for immunofluorescent staining for flow cytometry.
Application References
1. Ouchida, R., et al. 2010. J. Immunol. 185:294. PubMed 2. Chang, JH., et al. 2011. J. Immunol. 186:6999. PubMed.
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Description
This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may cross-react with other subclasses of mouse immunoglobulins.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.