The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
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Applications
FC - Quality tested
Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation, and blocking1 of the binding of ICAM-1 and fibrinogen. The CBRM1/5 antibody binds specifically to the activated form of CD11b. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301408).
Application References
1. Diamond M, et al. 1993. J. Cell Biol. 120:545. (Block) 2. Bryn T, et al. 2006. J. Immunol. 176:7361.J. Immunol. 3. Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200. 4. Barthel SR,et al.2006. Am J. Respir Cell Mol Biol.35:378. PubMed 5. Pillay J,et al. 2010. J. Leukocyte Biol. 88:211. PubMed 6. Wilson RP, et al. 2011. Infect Immun. 79:830. PubMed 7. Reis ES, et al. 2012. J. Immunol. 189:4797. PubMed.
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Description
The CBRM1/5 antibody reacts with an activated form of human CD11b, a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils, as well as adhesion, phagocytosis and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen and Factor X. The CBRM1/5 antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol-esters. This antibody does not recognize on-activated" CD11b. The epitope recognized by CBRM1/5 is contained in the I domain of the α chain. The CBRM1/5 antibody blocks cell adhesion to fibrinogen and ICAM-1.
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Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.